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#Post#: 20--------------------------------------------------
origin
By: gsgs Date: January 30, 2020, 9:53 pm
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HTML https://flutrackers.com/forum/forum/the-pandemic-discussion-forum/824572-discussion-chinese-academy-of-sciences-cas-in-wuhan-has-been-working-with-bats-and-coronavirus-for-many-years-dna-manipulations-cloning
[after WIV1 in 2012]
The sampling of this bat cave in Yunnan continued for another 5
years. From these samples,
the research team isolated 3 live viruses in succession, and
obtained the full-length genomic
sequences of a total of 15 bat SARS-like coronaviruses.
Surprisingly, the 15 strains contained
all the genome components of the SARS virus.
Hu Ben, an assistant researcher at the Wuhan Institute of
Virology, Chinese Academy of Sciences:
Shi Zhengli
...
fuchsia line
the highest similarity between SARS-like coronaviruses found in
bat caves and their respective
genes is above 97%,
After 13 years of virus tracing, the origin of the SARS virus
was finally found.
[ one of those Yunnan cave viruses must have been RaTG13 , 96%
similar to 2019-nCoV ]
#Post#: 21--------------------------------------------------
Re: origin
By: gsgs Date: January 30, 2020, 9:54 pm
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So far, the nCoV-2019 has been reported to share 96% sequence
identity to the RaTG13
genome (EPI_ISL_402131). However, the S1 Receptor Binding Domain
(RBD) of the nCoV-2019
genome was noticeably divergent between the two at amino acid
residues 350 to 550 – Figure 1A.
We aimed to identity coronaviruses related to nCoV-2019 in viral
metagenomics datasets available
in the public domain. In a recently published dataset describing
viral diversity in
Malayan pangolins (PRJNA573298 10) we used VirMAP 11 to
reconstruct a coronavirus genome
(approximately 84% complete from samples SRR10168377 2 and
SRR10168378 1) that
shared 97% amino acid identity across the same RBD segment –
Figure 1B. This result
indicates a potential recombination event for nCoV-2019.
pangolin=Schuppentier ,
HTML https://en.wikipedia.org/wiki/Pangolin
#Post#: 43--------------------------------------------------
Re: origin
By: gsgs Date: February 2, 2020, 6:42 pm
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Coronavirus conspiracy debunked by Wuhan researcher
By Zhang Zhihao | chinadaily.com.cn | Updated: 2020-02-02 19:29
A scientist from the Wuhan Institute of Virology of the Chinese
Academy of Sciences has
debunked a recent conspiracy which claimed the novel coronavirus
was manufactured and
escaped from the institute’s most advanced biocontainment
facility.
Shi Zhengli, a researcher from the institute, said on her social
media on Sunday the virus
was the result of "nature punishing the uncivilized habits and
customs of humans",
and she is willing to "bet my life that [the outbreak] has
nothing to do with the lab."
Shi was the leading scientist who made the discovery the novel
coronavirus can enter
cells through ACE2 receptors and may have originated from bats.
Her comments came after Indian scientists recently published a
non-peer reviewed
paper suggesting scientists had artificially inserted genes from
the HIV virus into the
coronavirus, thus making it susceptible to anti-HIV treatments.
The paper was immediately panned by experts around the world and
retracted from
biology journal server bioRxiv on Sunday.
...
HTML http://global.chinadaily.com.cn/a/202002/02/WS5e36b2b7a31012821727432e.ht
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ahh, "nature punishing the uncivilized habits and customs of
humans"
doesn't sound like a scientific, rational, logical arguing to me
...
BTW. the lab was not level IV before 2015
HTML https://en.wikipedia.org/wiki/Wuhan_Institute_of_Virology
#Post#: 48--------------------------------------------------
Re: origin
By: gsgs Date: February 4, 2020, 2:10 am
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mutation pictures of coronaviruses,
409 selected viruses from genbank,Dec.2019, close to SARS/nCoV
the ~30000 nucleotides were divided into areas of 3000
so they fit on one page
using my old program designed for influenza (which has segments
of <2300 nucleotides)
each black pixel is a nucleotide different from the average at
that position
each line is a virus, each column is a position
positions without mutation are left out
rows and columns are re-sorted for maximum connected areas of
black pixels
the average = orange rows are mainly human SARS-viruses
(I should remove them and run it again ...)
HTML http://magictour.free.fr/oro5f.GIF
#Post#: 64--------------------------------------------------
Re: origin
By: gsgs Date: February 7, 2020, 2:49 am
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South China Agricultural University finds pangolin as a
potential intermediate host
for a new coronavirus
Review and release: Chen Chenchen, the propaganda department
Source unit and
reviewer: Yang Zhiqun, propaganda department Time: 2020-02-07
Views: 5114
The latest research conducted by South China Agricultural
University, Lingnan Modern
Agricultural Science and Technology Guangdong Provincial
Laboratory,
Professor Shen Yongyi, and Professor Xiao Lihua, together with
Researcher Yang Ruiyu
from the Military Medical Research Institute of the Chinese
Academy of Military Sciences
and Chen Wu Senior Veterinarian of the Guangzhou Zoo Research
Department, shows
Pangolin is a potential intermediate host for a new coronavirus.
This latest discovery
will have great significance for the prevention and control of
the origin of the new coronavirus.
HTML https://www.scau.edu.cn/2020/0207/c1300a219015/page.htm
#Post#: 65--------------------------------------------------
Re: origin
By: epsilon Date: February 7, 2020, 5:20 am
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@gsgs: What is your opinion on the following article ?
HTML https://jameslyonsweiler.com/2020/02/02/moderately-strong-confirmation-of-a-laboratory-origin-of-2019-ncov/
Normally I am very sceptical about lab origin rumors and
wouldn't even bother reading if it were from a random internet
blog.
But the author seems to be a cerdible expert (PhD, author of
books and peer reviewed papers)
Excerpts:
"Unlike other related coronaviruses, the 2019-nCoV virus has a
unique sequence about 1,378 bp (nucleotide base pairs) long that
is not found in related coronaviruses."
"The gap in the line shows a lack of sequence homology beween
the most similar bat coronavirus and 2019-nCoV."
"The resulting massive alignment confirms a major unique
inserted element in 2019-nCoV not found in other bat
coronaviruses, nor in SARS in the homologous genomic position"
related:
HTML https://jameslyonsweiler.com/2020/01/30/on-the-origins-of-the-2019-ncov-virus-wuhan-china/
#Post#: 68--------------------------------------------------
Re: origin
By: gsgs Date: February 7, 2020, 9:52 am
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I'll check this later. I have no experience with coronaviruses,
but mainly influenza.
I already noticed, how close the envelope protein is and how
distant
the spike protein. But is it unusual ?
South China Agricultural University claimed today they have a
99% matching pangolin sequence
to be published soon
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OK, I selected 65 Wuhan-close coronaviruses, spike=protein-area
and made my mutation-picture:
HTML http://magictour.free.fr/spike6j.GIF
HTML http://magictour.free.fr/spike6j.l2
the ones close to each other in other areas are closer in the
spike-area too -
that looks to me as if it were just mutating much more in that
area -
rather than recombination.
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Pangolin paper from Sept.2019 :
HTML https://www.mdpi.com/1999-4915/11/11/979/htm
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