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       #Post#: 1493--------------------------------------------------
       (Abst.) Ocrelizumab vs. placebo, ocrelizumab vs. interferon
       beta-1a (NEJM)
   DIR By: agate
       Date: December 21, 2016, 6:36 pm
       ---------------------------------------------------------
       NEJM  (December 22, 2016) contains two articles on ocrelizumab
       in PPMS--one on ocrelizumab vs. placebo, the other on
       ocrelizumab vs. interferon beta-1a:
       --- Quote ---
       > Ocrelizumab versus Placebo in Primary Progressive Multiple
       Sclerosis
       >
       > Xavier Montalban, M.D., Stephen L. Hauser, M.D., Ludwig
       Kappos, M.D., Douglas L. Arnold, M.D., Amit Bar-Or, M.D.,
       Giancarlo Comi, M.D., Jérôme de Seze, M.D., Gavin Giovannoni,
       M.D., Hans-Peter Hartung, M.D., Bernhard Hemmer, M.D., Fred
       Lublin, M.D., Kottil W. Rammohan, M.D., Krzysztof Selmaj, M.D.,
       Anthony Traboulsee, M.D., Annette Sauter, Ph.D., Donna
       Masterman, M.D., Paulo Fontoura, M.D., Ph.D., Shibeshih
       Belachew, M.D., Ph.D., Hideki Garren, M.D., Ph.D., Nicole
       Mairon, M.D., Peter Chin, M.D., and Jerry S. Wolinsky, M.D., for
       the ORATORIO Clinical Investigators
       >
       > BACKGROUND
       >
       > An evolving understanding of the immunopathogenesis of
       multiple sclerosis suggests that depleting B cells could be
       useful for treatment. We studied ocrelizumab, a humanized
       monoclonal antibody that selectively depletes CD20-expressing B
       cells, in the primary progressive form of the disease.
       >
       > METHODS
       >
       > In this phase 3 trial, we randomly assigned 732 patients with
       primary progressive multiple sclerosis in a 2:1 ratio to receive
       intravenous ocrelizumab (600 mg) or placebo every 24 weeks for
       at least 120 weeks and until a prespecified number of confirmed
       disability progression events had occurred. The primary end
       point was the percentage of patients with disability progression
       confirmed at 12 weeks in a time-to-event analysis.
       >
       > B cells contribute to the pathogenesis of multiple sclerosis,
       including the primary progressive form.Although the mechanisms
       of tissue injury in multiple sclerosis are uncertain, B cells
       may influence pathogenesis through antigen presentation,6
       autoantibody production,or cytokine secretion.6 B cells are
       present in meningeal inflammation, which is characteristic of
       chronic multiple sclerosis and may cause adjacent cortical
       demyelinating and neurodegenerative pathologic features.CD20 is
       a cell-surface antigen found on pre-B cells and mature and
       memory B cells but not on the earliest B-cell precursors or on
       plasma cells.Ocrelizumab is a humanized monoclonal antibody that
       selectively depletes CD20-expressing B cells while preserving
       the capacity for B-cell reconstitution and preexisting humoral
       immunity.
       >
       > A previous phase 2–3 trial of the chimeric monoclonal
       anti-CD20 antibody rituximab (OLYMPUS trial) in primary
       progressive multiple sclerosis did not meet its primary efficacy
       end point, but a subgroup analysis showed delayed progression of
       disability in younger patients (<51 years of age) with evidence
       of increased inflammatory disease activity.3 Those results
       provided the rationale and in part informed the trial design for
       this investigation of ocrelizumab in patients with primary
       progressive multiple sclerosis. Here, we report results from a
       phase 3, randomized, parallel-group, double-blind,
       placebo-controlled trial (ORATORIO) that investigated the
       efficacy and safety of ocrelizumab in patients with primary
       progressive multiple sclerosis.
       >
       > RESULTS
       >
       > The percentage of patients with 12-week confirmed disability
       progression was 32.9% with ocrelizumab versus 39.3% with placebo
       (hazard ratio, 0.76; 95% confidence interval [CI], 0.59 to 0.98;
       P=0.03). The percentage of patients with 24-week confirmed
       disability progression was 29.6% with ocrelizumab versus 35.7%
       with placebo (hazard ratio, 0.75; 95% CI, 0.58 to 0.98; P=0.04).
       By week 120, performance on the timed 25-foot walk worsened by
       38.9% with ocrelizumab versus 55.1% with placebo (P=0.04); the
       total volume of brain lesions on T2-weighted magnetic resonance
       imaging (MRI) decreased by 3.4% with ocrelizumab and increased
       by 7.4% with placebo (P<0.001); and the percentage of
       brain-volume loss was 0.90% with ocrelizumab versus 1.09% with
       placebo (P=0.02). There was no significant difference in the
       change in the Physical Component Summary score of the 36-Item
       Short-Form Health Survey.
       >
       > Infusion-related reactions, upper respiratory tract
       infections, and oral herpes infections were more frequent with
       ocrelizumab than with placebo.
       >
       > Neoplasms occurred in 2.3% of patients who received
       ocrelizumab and in 0.8% of patients who received placebo; there
       was no clinically significant difference between groups in the
       rates of serious adverse events and serious infections.
       >
       > CONCLUSIONS
       >
       > Among patients with primary progressive multiple sclerosis,
       ocrelizumab was associated with lower rates of clinical and MRI
       progression than placebo. Extended observation is required to
       determine the long-term safety and efficacy of ocrelizumab.
       (Funded by F. Hoffmann–La Roche; ORATORIO ClinicalTrials.gov
       number, NCT01194570.)
       --- End Quote ---
       For discussion, see
  HTML http://www.nejm.org/doi/full/10.1056/NEJMoa1606468?query=TOC#t=articleDiscussion
  HTML http://www.nejm.org/doi/full/10.1056/NEJMoa1606468?query=TOC#t=articleDiscussion.
       The entire article can be seen here
  HTML http://www.nejm.org/doi/full/10.1056/NEJMoa1606468?query=TOC#t=articleTop.
       _________________________________________
       --- Quote ---
       > Ocrelizumab versus Interferon Beta-1a in Relapsing Multiple
       Sclerosis
       >
       > Stephen L. Hauser, M.D., Amit Bar-Or, M.D., Giancarlo Comi,
       M.D., Gavin Giovannoni, M.D., Hans-Peter Hartung, M.D., Bernhard
       Hemmer, M.D., Fred Lublin, M.D., Xavier Montalban, M.D., Kottil
       W. Rammohan, M.D., Krzysztof Selmaj, M.D., Anthony Traboulsee,
       M.D., Jerry S. Wolinsky, M.D., Douglas L. Arnold, M.D., Gaelle
       Klingelschmitt, Ph.D., Donna Masterman, M.D., Paulo Fontoura,
       M.D., Ph.D., Shibeshih Belachew, M.D., Ph.D., Peter Chin, M.D.,
       Nicole Mairon, M.D., Hideki Garren, M.D., Ph.D., and Ludwig
       Kappos, M.D., for the OPERA I and OPERA II Clinical
       Investigators
       >
       > BACKGROUND
       >
       > B cells influence the pathogenesis of multiple sclerosis.
       Ocrelizumab is a humanized monoclonal antibody that selectively
       depletes CD20+ B cells.
       >
       > METHODS
       >
       > In two identical phase 3 trials, we randomly assigned 821 and
       835 patients with relapsing multiple sclerosis to receive
       intravenous ocrelizumab at a dose of 600 mg every 24 weeks or
       subcutaneous interferon beta-1a at a dose of 44 &#956;g three
       times weekly for 96 weeks. The primary end point was the
       annualized relapse rate.
       >
       > RESULTS
       >
       > The annualized relapse rate was lower with ocrelizumab than
       with interferon beta-1a in trial 1 (0.16 vs. 0.29; 46% lower
       rate with ocrelizumab; P<0.001) and in trial 2 (0.16 vs. 0.29;
       47% lower rate; P<0.001).
       >
       > In prespecified pooled analyses, the percentage of patients
       with disability progression confirmed at 12 weeks was
       significantly lower with ocrelizumab than with interferon
       beta-1a (9.1% vs. 13.6%; hazard ratio, 0.60; 95% confidence
       interval [CI], 0.45 to 0.81; P<0.001), as was the percentage of
       patients with disability progression confirmed at 24 weeks (6.9%
       vs. 10.5%; hazard ratio, 0.60; 95% CI, 0.43 to 0.84; P=0.003).
       >
       > The mean number of gadolinium-enhancing lesions per
       T1-weighted magnetic resonance scan was 0.02 with ocrelizumab
       versus 0.29 with interferon beta-1a in trial 1 (94% lower number
       of lesions with ocrelizumab, P<0.001) and 0.02 versus 0.42 in
       trial 2 (95% lower number of lesions, P<0.001).
       >
       > The change in the Multiple Sclerosis Functional Composite
       score (a composite measure of walking speed, upper-limb
       movements, and cognition; for this z score, negative values
       indicate worsening and positive values indicate improvement)
       significantly favored ocrelizumab over interferon beta-1a in
       trial 2 (0.28 vs. 0.17, P=0.004) but not in trial 1 (0.21 vs.
       0.17, P=0.33).
       >
       > Infusion-related reactions occurred in 34.3% of the patients
       treated with ocrelizumab. Serious infection occurred in 1.3% of
       the patients treated with ocrelizumab and in 2.9% of those
       treated with interferon beta-1a. Neoplasms occurred in 0.5% of
       the patients treated with ocrelizumab and in 0.2% of those
       treated with interferon beta-1a.
       >
       >
       > CONCLUSIONS
       >
       > Among patients with relapsing multiple sclerosis, ocrelizumab
       was associated with lower rates of disease activity and
       progression than interferon beta-1a over a period of 96 weeks.
       Larger and longer studies of the safety of ocrelizumab are
       required. (Funded by F. Hoffmann–La Roche; OPERA I and II
       ClinicalTrials.gov numbers, NCT01247324 and NCT01412333,
       respectively.)
       --- End Quote ---
       The entire article can be seen here
  HTML http://www.nejm.org/doi/full/10.1056/NEJMoa1601277#t=articleTop.
       #Post#: 1494--------------------------------------------------
       B-cell depletion: A frontier in monoclonal antibodies for MS
   DIR By: agate
       Date: December 21, 2016, 6:45 pm
       ---------------------------------------------------------
       NEJM Editorial about these two articles (December 22, 2016) by
       Peter Calabresi, MD that gives a coherent summary of their
       results:
       --- Quote ---
       > EDITORIAL
       >
       > B-Cell Depletion — A Frontier in Monoclonal Antibodies for
       Multiple Sclerosis
       >
       > Peter A. Calabresi, M.D.
       >
       > Multiple sclerosis is a disabling autoimmune disease in which
       immune cells target central nervous system (CNS) antigens,
       leading to demyelination, glial activation, and subsequent loss
       of neurons and axons. There are three main subtypes of multiple
       sclerosis: relapsing–remitting, in which patients recover partly
       or fully from attacks but go on to have others; secondary
       progressive, in which patients with relapsing–remitting disease
       have progression of disability between attacks; and primary
       progressive, in which patients have continual progression from
       the time of onset of the disease.
       >
       > Over the past two decades, a remarkable number of new
       therapies have been developed that reduce the rate of relapses,
       reduce accumulation of lesions seen on magnetic resonance
       imaging (MRI), and modestly slow disability, but these are
       effective almost exclusively in relapsing multiple sclerosis.
       >
       > Most therapies for multiple sclerosis target T-cell
       activation, the trafficking of these cells into the CNS, and
       effector functions of the lymphocytes, but many have concomitant
       effects on B cells. Because B cells also migrate from the
       peripheral blood into the CNS in patients with multiple
       sclerosis and these cells produce immunoglobulins, which are a
       characteristic finding in the cerebrospinal fluid, several phase
       1b and 2 studies have tested the efficacy of the
       B-cell–depleting chimeric anti-CD20 monoclonal antibody
       rituximab. This therapy has reduced relapses and MRI activity in
       patients with relapsing–remitting multiple sclerosis but has not
       slowed the progression of disability in patients with primary
       progressive multiple sclerosis, except in a post hoc analysis of
       patients younger than 51 years of age and with
       gadolinium-enhancing lesions on MRI. Hauser et al. and Montalban
       et al. now report in the Journal the results of phase 3 trials
       of a new and fully humanized monoclonal anti-CD20 antibody,
       ocrelizumab, in two clinical trials in relapsing multiple
       sclerosis and one trial in primary progressive multiple
       sclerosis.
       >
       > In the identical trials involving patients with relapsing
       multiple sclerosis, called OPERA I and OPERA II, Hauser et al.
       compared ocrelizumab at a dose of 600 mg every 24 weeks versus
       interferon beta-1a at a dose of 44 &#956;g three times a week
       for 96 weeks. Both trials showed a significant effect of
       ocrelizumab on the primary outcome of annualized relapse rate,
       with ocrelizumab resulting in a 46% or 47% lower rate than with
       interferon beta-1a. The percentage of patients with disability
       progression and the number of lesions on MRI were also
       significantly lower in the ocrelizumab group. Remarkably, in the
       ORATORIO trial by Montalban et al. involving patients with
       primary progressive multiple sclerosis, a previously untreatable
       subtype of the disease, the relative risk of disability
       progression was approximately 25% lower among patients who
       received 600 mg of ocrelizumab every 24 weeks for at least 120
       weeks than among those who received placebo. In addition, the
       total volume of brain lesions on T2-weighted MRI decreased with
       ocrelizumab and increased with placebo. This is the first drug
       to show a significant effect in slowing disability progression
       in a phase 3 trial in primary progressive multiple sclerosis,
       and therefore the trial represents a landmark study in the
       field.
       >
       > The mechanism by which B-cell depletion achieves these effects
       is not fully understood but may be multifunctional, because B
       cells have important roles in antibody secretion, antigen
       presentation, and the release of effector cytokines. Although
       there are several other antigen-presenting cells, such as
       dendritic cells and monocytes, B cells by virtue of their number
       may be important antigen-presenting cells in multiple sclerosis.
       Previous studies have shown that rituximab rapidly reduced
       secretion of the inflammatory cytokines interferon-&#947; and
       interleukin-17 from T cells in patients with multiple sclerosis,
       findings that are consistent with the early reduction of active
       gadolinium-enhancing lesions observed with anti-CD20 therapy.
       >
       > In addition, the B cell is a reservoir for Epstein–Barr virus,
       which has been implicated in the pathogenesis of multiple
       sclerosis by virtue of its high sequence homology with myelin
       basic protein, and it is interesting to speculate that B-cell
       depletion may eliminate this reservoir, thereby decreasing
       autoreactivity, although this has not been proven.
       >
       > Primary progressive multiple sclerosis is characterized by
       insidious progression of disability over years with no remission
       and low MRI activity, and it is generally considered less
       inflammatory and more neurodegenerative than relapsing multiple
       sclerosis. These features are cited as explanations for the
       failure of other immunosuppressive drugs to decrease disease
       progression in primary progressive multiple sclerosis.
       >
       > One possible explanation for the positive effects of
       ocrelizumab in the ORATORIO trial is that the patient population
       included younger patients (mean age, approximately 45 years) and
       those with active MRI scans (>25% had gadolinium-enhancing
       lesions), allowing for a measurable antiinflammatory effect of
       ocrelizumab in patients who had some inflammation at an early,
       reversible stage of the disease.
       >
       > Another possible explanation is that B cells may mediate
       pathologic processes by secretion of cytokines or by deposition
       of immunoglobulins after they enter the CNS. B cells and plasma
       cells secrete antibodies that may target CNS antigens such as
       myelin, neurons, and glia, which could accelerate
       neurodegeneration or inhibit myelin repair. The continued
       separation of disability progression curves in the ORATORIO
       trial beyond 52 weeks, when antiinflammatory effects have been
       maximized, and success in the relatively noninflammatory
       disorder of primary progressive multiple sclerosis suggest that
       additional mechanisms of action may be operational, and further
       study is warranted.
       >
       > Although ocrelizumab offers promise for patients with primary
       progressive multiple sclerosis, who are desperately in need of a
       therapy, side effects must also be considered. Agents that
       target the immune system often result in some degree of immune
       suppression, potentially rendering the host susceptible to
       infections and impaired immune surveillance of new cancer cells,
       which could increase the risk of neoplasms.
       >
       > Although the dreaded complication of other drugs for multiple
       sclerosis, infection with JC virus causing progressive
       multifocal leukoencephalopathy, has not been seen with B-cell
       depletion in multiple sclerosis to date, there does appear to be
       a higher-than-normal risk of herpes reactivation and of
       neoplasms, especially breast cancer. These side effects will
       need to be studied in future trials and in phase 4 monitoring in
       the community to understand the extent of the risk. Clinicians
       are urged to carefully consider which patients might benefit the
       most from ocrelizumab and to stay vigilant with regard to
       monitoring for side effects that could be managed effectively if
       detected early.
       --- End Quote ---
       #Post#: 1501--------------------------------------------------
       Re: (Abst.) Ocrelizumab vs. placebo, ocrelizumab vs. interferon
       beta-1a (NEJM)
   DIR By: agate
       Date: December 26, 2016, 6:47 pm
       ---------------------------------------------------------
       This news has been selected as one of the 10 top neurology
       stories of 2016 by MedPage Today:
       --- Quote ---
       > MedPage Today asked specialists in neurology around the
       country to tell us what they thought were the most important
       clinical developments in 2016. These were the five most commonly
       mentioned.
       >
       > 1. Ocrelizumab for Primary Progressive MS
       >
       > Multiple sclerosis experts were hoping that a new drug that
       could treat not only relapsing MS, but also progressive disease,
       would be approved by the end of the year. That's not going to
       happen, since Genentech announced the FDA has pushed back the
       PDUFA date for ocrelizumab (Ocrevus) by 3 months as it reviews
       additional data on the company's manufacturing process.
       >
       > But hopes are high that the B-cell-targeting drug will be the
       first to win approval for progressive MS. Although there are
       several treatments for relapsing disease, no other drug has ever
       been approved to treat the more aggressive form.
       > Updated findings from the OPERA and ORATORIO studies were
       presented at various meetings throughout the year, and drugmaker
       Genentech has continued to tout the 24% reduced relative risk of
       disability progression in primary progressive MS, although some
       have expressed concern that its effects in PPMS are not as
       strong as in RRMS.
       >
       > "The excitement around the first effective therapy in PPMS is
       tempered by that benefit being predominantly in patients with
       active inflammation at the start of the trial, with little
       benefit seen among those without active inflammation at the
       start of the trial," said Robert Fox, MD, of the Cleveland
       Clinic. "To me, this trial suggests that patients with
       progressive MS and active inflammation may benefit from an
       anti-inflammatory therapy, but a treatment for the gradual,
       insidious progression seen in the majority of progressive MS
       patients remains elusive."
       >
       > Jerry Wolinsky, MD, of UTHealth and Memorial Hermann in
       Houston, said that even if ocrelizumab isn't approved for PPMS,
       "the effects of the drug in relapsing forms of the disease are
       impressive, and have refocused the field into considering B
       cells as contributing more to the immunopathogenesis of the
       disease than we have in the past."
       --- End Quote ---
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