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       #Post#: 2706--------------------------------------------------
       volume and concentration question
   DIR By: gusdub
       Date: October 21, 2013, 12:49 pm
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       Please help.
       I haven't taken physical chemistry yet (that's next term) but I
       am required to use that knowledge for something I'm doing now. I
       am supposed to design an experiment and it requires that I know
       c1v1=c2v2 but it totally isn't working for me. I must be missing
       something big.
       My problem. I want to make a caffeine solution as a treatment
       and my control is already made (no caffeine).
       for my control I am using 150g of sugar with 370 ml of distilled
       water. This is suppposed to give me a 30% sugar solution.
       for my treatments I want to make 0.1 M caffeine solution, 0.01 M
       and 0.001 M
       I got this advice for a friend but again not helping...
       For a target Caffeine concentration of 0.1mM we first make a
       stock solution with 1g of Caffeine in 1L of distilled water, and
       then we pipette 0.0073277 L from this into a 0.37L sucrose
       solution (regular 30% sugar water solution). In order to adjust
       for the additional volume, we also add an additional 2.9g of
       sugar to the sucrose/caffeine mixture solution. This was all
       calculated using C1V1=C2V2 formula. I'm not sure what the values
       would be for 0.01mM caffeine but you guys can figure that out.
       I know I looked at caffeine molarity and its 194.14 g per M ...
       but I need to adjust for the sugar solution to be 30% across all
       treatments...how do I do this? p.s. I need to make about 1 L
       worth of this solution and then I'll distribute it to 4 small
       vials.
       Please any help is greatly appreciated.
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