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#Post#: 1590--------------------------------------------------
SPECTROPHOTOMETRIC ESTIMATION OF SULPHATE AND SULFIDE
DIR By: sumaira aslam
Date: December 15, 2012, 10:55 am
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Hi,
Please i have a question how we can estimate sulphate and
sulfide formed in bacterial culture (sulphate reducing bacteria)
by simple spectrophotometric method? Even when sulfide formation
is also there (black ppt)?
#Post#: 1607--------------------------------------------------
Re: SPECTROPHOTOMETRIC ESTIMATION OF SULPHATE AND SULFIDE
DIR By: Michel
Date: December 17, 2012, 7:09 pm
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Hi..
How about this:
HTML http://www.sciencedirect.com/science/article/pii/0003269778903846
---Quote---
A simple, rapid, and sensitive procedure for the study of
sulfatase kinetics using nonchromogenic substrates is described.
The procedure utilizes the dye potassium rhodizonate which forms
a complex with barium ions resulting in a pink color. The extent
of reduction of the pink color in the presence of sulfate ions
indicates the quantity of sulfate
---End Quote---
#Post#: 1624--------------------------------------------------
Re: SPECTROPHOTOMETRIC ESTIMATION OF SULPHATE AND SULFIDE
DIR By: sumaira aslam
Date: December 18, 2012, 10:33 am
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Thank you Shiva for sharing this information but will you please
give me another favour? I have no access to full text of article
can you please attach me the full article?
#Post#: 1629--------------------------------------------------
Re: SPECTROPHOTOMETRIC ESTIMATION OF SULPHATE AND SULFIDE
DIR By: Shiva
Date: December 19, 2012, 8:29 am
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haha Sumaira, actually Michel answered your question not me :-)
Hope he will help you in accessing the full link.
Thanks,
Shiva
#Post#: 1631--------------------------------------------------
Re: SPECTROPHOTOMETRIC ESTIMATION OF SULPHATE AND SULFIDE
DIR By: Michel
Date: December 19, 2012, 12:16 pm
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Sadly, the book is not free...I do not have docs to consult,
also I dont think I have any friends that's expert in this area.
I have to announce this topic, Sumaira. Hopefully someone
knowledgeable in this eield will reply.
#Post#: 1633--------------------------------------------------
Re: SPECTROPHOTOMETRIC ESTIMATION OF SULPHATE AND SULFIDE
DIR By: Shiva
Date: December 19, 2012, 12:23 pm
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Yep, "SPECTROPHOTOMETRIC ESTIMATION" makes me stay away from
this topic haha. Well, sorry, I've no idea either :(
Shiva
#Post#: 1658--------------------------------------------------
Re: SPECTROPHOTOMETRIC ESTIMATION OF SULPHATE AND SULFIDE
DIR By: sumaira aslam
Date: December 21, 2012, 11:01 am
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--- Quote from: Michel link ---
>
> Sadly, the book is not free...I do not have docs to consult,
also I dont think I have any friends that's expert in this area.
> I have to announce this topic, Sumaira. Hopefully someone
knowledgeable in this eield will reply.
>
--- End Quote ---
Thanks Michel for correspondence
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