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       #Post#: 113--------------------------------------------------
       Chemically defined generation of human cardiomyocytes
       By: SSamani Date: March 10, 2015, 1:10 am
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       [font=verdana]
       [center]Chemically defined generation of human
       cardiomyocytes[b][/center]
       [center]Nature Methods Vol 11, Issue 8, 2014
       (DOI:10.1038/nmeth.2999)[/center]
       Existing methods for human induced pluripotent stem cell (hiPSC)
       cardiac differentiation are efficient but require complex,
       undefined medium constituents that hinder further elucidation of
       the molecular mechanisms of cardiomyogenesis. Using hiPSCs
       derived under chemically defined conditions on synthetic
       matrices, we systematically developed an optimized cardiac
       differentiation strategy, using a chemically defined medium
       consisting of just three components: the basal medium RPMI 1640,
       L-ascorbic acid 2-phosphate and rice-derived recombinant human
       albumin. Along with small molecule–based induction of
       differentiation, this protocol produced contractile sheets of up
       to 95% TNNT2+ cardiomyocytes at a yield of up to 100
       cardiomyocytes for every input pluripotent cell and was
       effective in 11 hiPSC lines tested. This chemically defined
       platform for cardiac specification of hiPSCs will allow the
       elucidation of cardiomyocyte macromolecular and metabolic
       requirements and will provide a minimal system for the study of
       maturation and subtype specification.
       [center][b][size=12pt]Download link
  HTML http://www.uplooder.net/cgi-bin/dl.cgi?key=e426918ec25b53da4b5478d17f7b1e8f[/center]
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