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#Post#: 87--------------------------------------------------
Corneal repair by iPSCs and amphiphatic chitosan hydrogel
By: SSamani Date: February 21, 2015, 2:16 pm
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[font=verdana][center]Corneal repair by human corneal
keratocyte-reprogrammed iPSCs and amphiphatic
carboxymethyl-hexanoyl chitosan hydrogel
Biomaterials 33 (2012) 8003-8016[/center]
Abstract
Induced pluripotent stem cells (iPSCs) have promising potential
in regenerative medicine, but whether iPSCs can promote corneal
reconstruction remains undetermined. In this study, we
successfully reprogrammed human corneal keratocytes into iPSCs.
To prevent feeder cell contamination, these iPSCs were cultured
onto a serum- and feeder-free system in which they remained
stable through 30 passages and showed ESC-like pluripotent
property. To investigate the availability of iPSCs as
bioengineered substitutes in corneal repair, we developed a
thermo-gelling injectable amphiphatic carboxymethyl-hexanoyl
chitosan (CHC) nanoscale hydrogel and found that such gel
increased the viability and CD44+proportion of iPSCs, and
maintained their stem-cell like gene expression, in the presence
of culture media. Combined treatment of iPSC with CHC hydrogel
(iPSC/CHC hydrogel) facilitated wound healing in surgical
abrasion-injured corneas. In severe corneal damage induced by
alkaline, iPSC/CHC hydrogel enhanced corneal reconstruction by
downregulating oxidative stress and recruiting endogenous
epithelial cells to restore corneal epithelial thickness.
Therefore, we demonstrated that these human
keratocyte-reprogrammed iPSCs, when combined with CHC hydrogel,
can be used as a rapid delivery system to efficiently enhance
corneal wound healing. In addition, iPSCs reprogrammed from
corneal surgical residues may serve as an alternative cell
source for personalized therapies for human corneal damage.
[center][size=12pt]Download link
HTML http://www.uplooder.net/cgi-bin/dl.cgi?key=9bb289049982f5c50303fbc43602921e[/center]
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