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       #Post#: 57--------------------------------------------------
       Stem Cells Differentiation into Retinal Ganglion-Like Cells
       By: nourani Date: January 2, 2015, 2:00 am
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       [center]Dental Pulp Stem Cells Differentiation into Retinal
       Ganglion-Like Cells in a Three Dimensional Network[/center]
       Roozafzoon R1, Lashay A2, Vasei M3, Ai J4, Khoshzaban A5, Keshel
       SH2, Bahrami H2
       ABSTRACT The loss of retinal ganglion cells (RGCs) in majority
       of retinal degenerative diseases is the first seen pathological
       event. A lot of studies aim to discover suitable cell sources to
       replace lost and damaged RGCs. Among them dental pulp stem cells
       (DPSCs) have a great potential of differentiating into neuronal
       lineages as well as RGCs. Moreover, three-dimensional (3D)
       networks and its distribution for growing and differentiation of
       stem cells as much as possible mimic to native tissue holds
       great potential in retinal tissue engineering. In this study, we
       isolate DPSCs from rat incisors and validate them with flow
       cytometry. Briefly, we differentiated cells using DMEM/F12
       containing FGF2, Shh and 0.5% FBS into retinal ganglion-like
       cells (RGLCs) in two conditions; 3D state in biocompatible
       fibrin hydrogel and two-dimensional (2D) or conventional culture
       in polystyrene plates. Immuncytochemical and gene expression
       analysis revealed the expression of Pax6, Atoh7 and BRN3B
       increased in 3D fibrin culture compared to 2D conventional
       culture. In combination, these data demonstrate that using 3D
       networks can resemble near natural tissue properties for
       effective generating RGCs which used to treat neurodegenerative
       diseases such as glaucoma.
       Biochem Biophys Res Commun. 2014 Dec 24. pii:
       S0006-291X(14)02251-7. doi: 10.1016/j.bbrc.2014.12.069. [Epub
       ahead of print]
       #Post#: 74--------------------------------------------------
       Re: Stem Cells Differentiation into Retinal Ganglion-Like Cells
       By: SSamani Date: February 2, 2015, 9:25 am
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       [center][font=verdana]Full paper.[/font][/center]
       [center]Download link
  HTML http://www.uplooder.net/cgi-bin/dl.cgi?key=f384e15d1586e0e45367566757165d68[/center]
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