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#Post#: 28--------------------------------------------------
Bioglass application in Peripheral Nerve Regeneration
By: nourani Date: December 17, 2014, 7:47 pm
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Development of a Nerve Growth Conduit for Peripheral Nerve
Regeneration
Mohammad Reza Nourani1*
1Tissue Engineering Research Center, Applied Biotechnology
Research Center, Baqiyatallah University of Medical Sciences,
Tehran, Iran
*Corresponding author: Mohammad Reza Nourani, PhD, Tissue
Engineering Research Center, Applied Biotechnology Research
Center, Baqiyatallah Hospital, Baqiyatallah University of
Medical Sciences, Mollasadra Ave, Tehran, Iran. Tel: +98 21 8821
1523; Fax: +98 21 8821 1524; E-mail: r.nourani@yahoo.com
In peripheral nerve injuries, the gap between the proximal and
distal ends of the injured nerve should be bridged by a
tube-shape implant in order to guide the sprouts sent out by the
proximal end to the distal end. Application of both autografts
and allografts are limited; the former by availability of donor
tissue, donor site morbidity and formation of neuroma; and the
latter by its low potential in promotion of nerve regeneration
and the risk of transmission of pathogens. Therefore, conduits
made of different biomaterials have been developed to regenerate
a nerve gap wider than 10 mm. In this study, different
biomaterial solutions were prepared. Then, a mandrel was soaked
in the solutions and freeze-dried. The conduits underwent SEM
for assessment of pores size and surface morphology and FTIR for
evaluation of chemical structure. Also, MTT assay was performed
using rat mesenchymal stem cell lines to test for cytotoxicity.
A final histopathological study was performed too. The results
showed that the conduits had a highly porous fine structure with
interconnected and partly equal-sized pores. FTIR spectra showed
presence of ordinary functional groups. In vitro studies
confirmed the ability of the conduits to support growth of
fibroblast cells and histopathological study revealed the
growing processes of nerve fibers from the proximal end of the
cut nerve to its distal end. It could be concluded that the
nerve growth conduit is suitable for guiding axonal growth and
supporting peripheral nerve regeneration. In this presentation,
different types of nerve conduits are explained.
HTML http://onlinelibrary.wiley.com/doi/10.1111/aor.12136/full
HTML http://onlinelibrary.wiley.com/doi/10.1111/aor.12136/full
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